CD4+CD25+ regulatory T cells inhibit natural killer cell functions in a transforming growth factor–β–dependent manner

نویسندگان

  • François Ghiringhelli
  • Cédric Ménard
  • Magali Terme
  • Caroline Flament
  • Julien Taieb
  • Nathalie Chaput
  • Pierre E. Puig
  • Sophie Novault
  • Bernard Escudier
  • Eric Vivier
  • Axel Lecesne
  • Caroline Robert
  • Jean-Yves Blay
  • Jacky Bernard
  • Sophie Caillat-Zucman
  • Antonio Freitas
  • Thomas Tursz
  • Orianne Wagner-Ballon
  • Claude Capron
  • William Vainchencker
  • François Martin
  • Laurence Zitvogel
چکیده

Tumor growth promotes the expansion of CD4+CD25+ regulatory T (T reg) cells that counteract T cell-mediated immune responses. An inverse correlation between natural killer (NK) cell activation and T reg cell expansion in tumor-bearing patients, shown here, prompted us to address the role of T reg cells in controlling innate antitumor immunity. Our experiments indicate that human T reg cells expressed membrane-bound transforming growth factor (TGF)-beta, which directly inhibited NK cell effector functions and down-regulated NKG2D receptors on the NK cell surface. Adoptive transfer of wild-type T reg cells but not TGF-beta-/- T reg cells into nude mice suppressed NK cell-mediated cytotoxicity, reduced NKG2D receptor expression, and accelerated the growth of tumors that are normally controlled by NK cells. Conversely, the depletion of mouse T reg cells exacerbated NK cell proliferation and cytotoxicity in vivo. Human NK cell-mediated tumor recognition could also be restored by depletion of T reg cells from tumor-infiltrating lymphocytes. These findings support a role for T reg cells in blunting the NK cell arm of the innate immune system.

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عنوان ژورنال:
  • The Journal of Experimental Medicine

دوره 202  شماره 

صفحات  -

تاریخ انتشار 2005